Plasminogen Activator Inhibitor Type 1 Gene Polymorphism in Patients with Minimal Change Nephrotic Syndrome

Kim, Young-Min; Hong, Hyun-Kee; Kim, Sung-Do; Cho, Byoung-Soo

J Korean Soc Pediatr Nephrol > Volume 8(1); 2004 > Article

J Korean Soc Pediatr Nephrol 2004;8(1): 26-32.

소아 신증후군 환자에서 Plasminogen Activator Inhibitor Type 1 유전자 다형성

김영민, 홍현기, 김성도, 조병수

¹경희대학교 의과대학 소아과교실
²경희대학교 의과대학 소아과교실
³경희대학교 의과대학 동서신장병연구소
⁴경희대학교 의과대학 동서신장병연구소

Plasminogen Activator Inhibitor Type 1 Gene Polymorphism in Patients with Minimal Change Nephrotic Syndrome

Young-Min Kim, Hyun-Kee Hong, Sung-Do Kim, Byoung-Soo Cho

¹School of Medicine, Department of Pediatrics, College of Medicine, Kyung-Hee University
²School of Medicine, Department of Pediatrics, College of Medicine, Kyung-Hee University
³East-West Kidney Disease Research Institute, College of Medicine, Kyung-Hee University
⁴East-West Kidney Disease Research Institute, College of Medicine, Kyung-Hee University

ABSTRACT

Purpose : Hypercoagulability is present in patients with nephrotic syndrome. Plasminogen activator inhibitor type 1(PAI-1) is a major inhibitor of plasminogen activators. PAI-1 inactivates both tissue plasminogen activator(tPA) and urokinase plasminogen activator(uPA) by rapid formation of inactive 1:1 stoichiometric complexes. Recently some studies showed that the enhanced PAI-1 expression may be involved in the intraglomerular fibrinogen/fibrinrelated antigen deposition seen in nephrotic syndrome.
Methods : PAI-1 gene promoter -844(G/A) polymorphism was evaluated in 146 children with minimal change nephrotic syndrome(MCNS) and 230 control subjects. The patients with MCNS were subdivided into 85 infrequent-relapser(IR) group and 61 frequent relapser(FR) group. PCR of PAI-1 gene promoter region including -844(G/A) and RFLP using the restriction enzyme Xhol were performed for each DNA samples extracted from the groups.
Results : The distribution of PAI-1 genotype in the control group was G/G 81(32.5%), A/A 42(16.9%), and G/A 126(50.6%). The distribution of PAI-1 genotypes in the IR group of MCNS was G/G 29(34.1%), A/A 15(17.7%), and G/A 41(48.2%). The distribution of PAI-1 genotype in the FR group of MCNS was G/G 17(27.9%), A/A 18(29.5%), and G/A 26(42.6%). There was a significantly increased frequency of A/A genotype(P=0.0251) in the FR group of MCNS.
Conclusion : Our results indicate that the PAI-1 gene promoter A/A genotype may be associated with the FR in MCNS.

Key words: Plasminogen activator inhibitor type 1 | Polymorphism | Nephrotic Syndrome