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Journal of the Korean Society of Pediatric Nephrology 2009;13(2): 170-175. doi: https://doi.org/10.3339/jkspn.2009.13.2.170
단백질체학을 이용하여 국소성 분절성 사구체 경화증과 미세 변화형 신증후군의 비교
김성도, 조병수
1경희대학교 의과대학 소아과학교실
2경희대학교 의과대학 소아과학교실
The Comparison between FSGS and MCNS Using Proteomic Method in Childhood Nephrotic Syndrome; Preliminary Study
Sung-Do Kim, Byoung-Soo Cho
1Department of Pediatrics, School of Medicine, Kyung Hee University
2Department of Pediatrics, School of Medicine, Kyung Hee University
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ABSTRACT
Purpose : FSGS do not respond well to any kind of therapy and gradually progress to end-stage renal disease. This study was conducted to investigate the difference of protein expression between MCNS and FSGS as a preliminary study for understanding the pathophysiology of FSGS.
Methods : Renal biopsy samples of MCNS and FSGS were obtained, which was diagnosed by one pathologist. They were solubilized with a conventional extraction buffer for protein extraction. The solution was applied on immobilized linear gradient strip gel (pH 4-7) using IPGphor system. Silver staining was carried out according to standard method. Protein identification was done by searching NCBI database using MASCOT Peptide Mass Fingerprint software.
Results : The differences in protein expressions between MCNS and FSGS were shown by increased or decreased protein spots. Most prominently expressed spot among several spots in FSGS was isolated and analyzed, one of which was glutathione S-transferase (GST) P1-1, whereas it was not found in MCNS. So GSTP1-1 was considered as the one of the key biomarkers in pathogenesis of FSGS.
Conclusion : This result would be helpful in diagnosing FSGS and researching FSGS. Further studies for glutathione S-transferase P1-1 might be necessary to elucidate the mechanisms regarding FSGS.
Key words: Nephrotic syndrome | Glomerulosclerosis | Proteomics | Glutathione S-transferase
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