J Korean Soc Pediatr Nephrol > Volume 17(2); 2013 > Article
J Korean Soc Pediatr Nephrol 2013;17(2): 79-85. doi: https://doi.org/10.3339/jkspn.2013.17.2.79
Puromycin aminonucleoside의 사구체 족세포 Pcadherin에 대한 영향
충북대학교 의과대학 소아과학교실
Effect of Puromycin Aminonucleoside on Podocyte P-Cadherin
Tae-Sun Ha
Department of Pediatrics, College of Medicine, Chungbuk National University, Cheongju, Korea
Corresponding Author: Tae-Sun Ha ,Tel: 043-269-6374, Fax: 043-264-6620, Email: tsha@chungbuk.ac.kr
Received: September 13, 2013;  Accepted: October 7, 2013.
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http:// creativecommons. org/licenses/bync/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Purpose: To test whether the expression of P-cadherin, a component of slit diaphragms between podocyte foot processes, would be altered by puromycin aminonucleoside (PAN) in a cultured podocyte in vitro.

Rat glomerular epithelial cells (GEpC) were cultured with various concentrations of PAN. The distribution of P-cadherin was examined with a confocal microscope. Western blotting and reverse transcriptase-polymerase chain reaction (RT-PCR) were used to measure the change in P-cadherin expression.

Results :
This study found that P-cadherin was concentrated in the inner and peripheral cytoplasm with high concentrations of PAN under immunofluorescence views. Western blotting of GEpC revealed that PAN induced a decrease of P-cadherin in dose- and time-dependent manners. A high dose (50 μg/mL) of PAN decreased P-cadherin expression by 21.9% at 24 h (P <0.05) and 31.9% at 48 h (P <0.01) compared to those without PAN. In RT-PCR, high concentrations (50 μg/mL) of PAN also decreased P-cadherin mRNA expression, similar to protein suppression, by 23.5% at 48 h (P <0.05).

Conclusion :
Podocytes exposed to PAN in vitro concentrated P-cadherin internally, and reduced P-cadherin mRNA and protein expression. This could explain the development of proteinuria in experimental PAN-induced nephropathy.
Key words: P-Cadherin | Puromycin aminonucleoside (PAN)-induced nephropathy | Glomerular epithelial cells (GEpC) | Podocyte
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