J Korean Soc Pediatr Nephrol > Volume 6(1); 2002 > Article
Journal of the Korean Society of Pediatric Nephrology 2002;6(1): 75-84.
실험적 급성 신우신염이 유발된 이유기 백서에서 methylprednisolone이 TGF-${beta}1$, 세포고사 및 신반흔에 미치는 영향
황수자, 성순희, 이승주
1이화여자대학교 의과대학 소아과학교실
2이화여자대학교 의과대학 병리학교실
3이화여자대학교 의과대학 소아과학교실
Effects of Methylprednisolone on TGF-${beta}1$, Apoptosis and Renal Scarring in Experimental Acute Pyelonephritic Weaning Rats
Soo-Ja Whang, Soon-Hee Sung, Seung-Joo Lee
1Department of Pediatrics, Ewha Womans University, College of Medicine
2Department of Pathology, Ewha Womans University, College of Medicine
3Department of Pediatrics, Ewha Womans University, College of Medicine
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ABSTRACT
Purpose Acute pyelonephritis of growing kidneys may result in renal scarring. TGF-${beta}1$, inflammatory cytokine, has been suggested to play an important role in promoting renal scarring through apoptosis, suppression of cellular proliferation and fibrosis. We observed the effects of a potent anti-inflammatory agent, methylprednisolone on apoptosis and renal scarring in experimentally induced acute pyelonephritic weaning rats. Materials and
Methods: To induce ascending pyelonephritis a saline solution containing Escherichia coli type ATCC No. 25922, pili- form (107 bacteria/mL) was infused into the bladder through the 16-guage silicone cannula for 48 hours to 102 three-week-old Sprague-Dawley rats (50-60g). Experimental groups were divided into three groups according to the treatment protocols, group I (ceftriaxone only, n=3l), group II (methylprednisolone+ceftriaxone n=28), control group (n=43) was not treated. Histopathologic scores of inflammatory changes, fibrosis and tubular atrophy, the apoptosis index and TGF-${beta}1$ expression score were observed at post-infection 1 and 3 week. Datas were analysed using ANOVA test and P value below 0.05 was interpreted as significant.
Results : The mortality rate ($21.4%$) of group II was not different to the control group ($41.9%$) and group I ($32.3%$). The inflammatory score of group II ($0.8{pm}0.87$) at week 1 was significantly lower than those of the control group ($2.3{pm}0.87$) and Group I ($1.7{pm}0.79$) (P<0.05). Apoptosis index of group II ($2.9{pm}2.15$) at week 1 was significantly lower than those of the control group ($10.0{pm}1.95$) and group 1 ($8.3{pm}2.53$) (P<0.05). TCF-${beta}1$ expression score of group II ($0.8{pm}0.72$) at week 1 was significantly lower than those of the control group ($1.9{pm}0.68$) and group I ($1.8{pm}0.60$) (P<0.05). The fibrosis score of group II ($1.1{pm}1.10$) at week 3 was significantly lower than that of the group I ($1.8{pm}0.83$) (P<0.05)
Conclusion : Conclusion Combined treatment with methylprednisolone and ceftriaxone reduced inflammation, fibrosis, apoptosis and TGF-${beta}1$ expression in acute pyelonephritic weaning rats, compared to ceftriaxone alone. Anti-inflammatory agent supplemented to antibiotics could prevent renal scarring more effectively. (J Korean Soc Pediatr Nephrol 2002 ; 6 : 75-84)
Key words: methylprednisolone | apoptosis | renal scar | pyelonephritic weaning rat
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